Dynamic alterations in chemokine gradients induce transendothelial shuttling of human T cells under physiologic shear conditions.

ABSTRACT: The active movement of cells from subendothelial compartments into the bloodstream (intravasation) has been recognized for several decades by histologic and physiologic studies, yet the molecular effectors of this process are relatively uncharacterized. For extravasation, studies based predominantly on static transwell assays support a general model, whereby transendothelial migration (TEM) occurs via chemoattraction toward increasing chemokine concentrations. However, this model of chemotaxis cannot readily reconcile how chemokines influence intravasation, as shear forces of blood flow would likely abrogate luminal chemokine gradient(s). Thus, to analyze how T cells integrate perivascular chemokine signals under physiologic flow, we developed a novel transwell-based flow chamber allowing

SUBMITTER: Lee JY 

PROVIDER: S-EPMC2796626 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature