Kinetic simulation of a centrifugal bioreactor for high population density hybridoma culture.

ABSTRACT: Demand for increasingly complex post-translationally modified proteins, such as monoclonal antibodies (mAbs), necessitates the use of mammalian hosts for production. The focus of this article is a continuous centrifugal bioreactor (CCBR) capable of increasing volumetric productivity for mAb production through high density hybridoma culture, exceeding 10(8) cells/mL. At these extreme densities, environmental conditions such as substrate and inhibitor concentrations rapidly change dramatically affecting the growth rate. The development of a kinetic model predicting glucose, mAb, lactate, and ammonium concentrations based on dilution rate and cell density is shown in this article. Additionally, it is found that pH affects both growth rate and viability, and a range of 6.9-7.4 is needed to mai

SUBMITTER: Detzel CJ 

PROVIDER: S-EPMC2796705 | biostudies-literature | 2009 Nov-Dec

REPOSITORIES: biostudies-literature