Lung dendritic cell expression of maturation molecules increases with worsening chronic obstructive pulmonary disease.

ABSTRACT:

Rationale

Dendritic cells (DCs) have not been well studied in chronic obstructive pulmonary disease (COPD), yet their integral role in activating and differentiating T cells makes them potential participants in COPD pathogenesis.

Objectives

To determine the expression of maturation molecules by individual DC subsets in relationship to COPD stage and to expression of the acute activation marker CD69 by lung CD4(+) T cells.

Methods

We nonenzymatically released lung leukocytes from human surgical specimens (n = 42) and used flow cytometry to identify three DC subsets (mDC1, mDC2, and pDC) and to measure their expression of three costimulatory molecules (CD40, CD80 and CD86) and of CD83, the definitive marker of DC maturation. Spearman nonparametric correlation analysis w

SUBMITTER: Freeman CM 

PROVIDER: S-EPMC2796731 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature