Identification of microRNA activity by Targets' Reverse EXpression.

ABSTRACT:

Motivation

Non-coding microRNAs (miRNAs) act as regulators of global protein output. While their major effect is on protein levels of target genes, it has been proven that they also specifically impact on the messenger RNA level of targets. Prominent interest in miRNAs strongly motivates the need for increasing the options available to detect their cellular activity.

Results

We used the effect of miRNAs over their targets for the detection of miRNA activity using mRNAs expression profiles. Here we describe the method, called T-REX (from Targets' Reverse EXpression), compare it to other similar applications, show its effectiveness and apply it to build activity maps. We used six different target predictions from each of four algorithms: TargetScan, PicTar, DIANA-microT and DI

SUBMITTER: Volinia S 

PROVIDER: S-EPMC2796810 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature