Biosynthetic studies of aziridine formation in azicemicins.

ABSTRACT: The azicemicins, which are angucycline-type antibiotics produced by the actinomycete, Kibdelosporangium sp. MJ126-NF4, contain an aziridine ring attached to the polyketide core. Feeding experiments using [1-(13)C]acetate or [1,2-(13)C(2)]acetate indicated that the angucycline skeleton is biosynthesized by a type II polyketide synthase. Isotope-tracer experiments using deuterium-labeled amino acids revealed that aspartic acid is the precursor of the aziridine moiety. Subsequent cloning and sequencing efforts led to the identification of the azicemicin (azic) gene cluster spanning approximately 50 kbp. The cluster harbors genes typical for type II polyketide synthesis. Also contained in the cluster are genes for two adenylyl transferases, a decarboxylase, an additional acyl carrier protein (

SUBMITTER: Ogasawara Y 

PROVIDER: S-EPMC2796913 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature