Environmental DNA sequencing primers for eutardigrades and bdelloid rotifers.

ABSTRACT:

Background

The time it takes to isolate individuals from environmental samples and then extract DNA from each individual is one of the problems with generating molecular data from meiofauna such as eutardigrades and bdelloid rotifers. The lack of consistent morphological information and the extreme abundance of these classes makes morphological identification of rare, or even common cryptic taxa a large and unwieldy task. This limits the ability to perform large-scale surveys of the diversity of these organisms.Here we demonstrate a culture-independent molecular survey approach that enables the generation of large amounts of eutardigrade and bdelloid rotifer sequence data directly from soil. Our PCR primers, specific to the 18s small-subunit rRNA gene, were developed for both eutar

SUBMITTER: Robeson MS 

PROVIDER: S-EPMC2796995 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature