Alterations of the mitochondrial proteome caused by the absence of mitochondrial DNA: A proteomic view.

ABSTRACT: The proper functioning of mitochondria requires that both the mitochondrial and the nuclear genome are functional. To investigate the importance of the mitochondrial genome, which encodes only 13 subunits of the respiratory complexes, the mitochondrial rRNAs and a few tRNAs, we performed a comparative study on the 143B cell line and on its Rho-0 counterpart, i.e., devoid of mitochondrial DNA. Quantitative differences were found, of course in the respiratory complexes subunits, but also in the mitochondrial translation apparatus, mainly mitochondrial ribosomal proteins, and in the ion and protein import system, i.e., including membrane proteins. Various mitochondrial metabolic processes were also altered, especially electron transfer proteins and some dehydrogenases, but quite often on a fe

SUBMITTER: Chevallet M 

PROVIDER: S-EPMC2797067 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature