Signalling with retinoids in the human lung: validation of new tools for the expression study of retinoid receptors.

ABSTRACT:

Background

Retinoid Receptors are involved in development and cell homeostasis. Alterations of their expressions have been observed in lung cancer. However, retinoid chemoprevention trials in populations at risk to develop such tumors have failed. Therefore, the pertinence of new clinical trials using second generation retinoid requires prior better understanding of retinoid signalling. This is our aim when validating extensively research tools, focused on Retinoic Acid Receptor beta, whose major role in lung cancer is documented.

Methods

Biocomputing was used to assess the genomic organization of RAR beta. Its putative RAR-beta1' promoter features were investigated experimentally. Specific measures realized, with qRT-PCR Syber Green assays and a triplex of Taqman probes, we

SUBMITTER: Poulain S 

PROVIDER: S-EPMC2797528 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature