Improved mass spectrometry compatibility is afforded by ammoniacal silver staining.

ABSTRACT: Sequence coverage in MS analysis of protein digestion-derived peptides is a key issue for detailed characterization of proteins or identification at low quantities. In gel-based proteomics studies, the sequence coverage greatly depends on the protein detection method. It is shown here that ammoniacal silver detection methods offer improved sequence coverage over standard silver nitrate methods, while keeping the high sensitivity of silver staining. With the development of 2D-PAGE-based proteomics, another burden is placed on the detection methods used for protein detection on 2-D-gels. Besides the classical requirements of linearity, sensitivity, and homogeneity from one protein to another, detection methods must now take into account another aspect, namely their compatibility with MS. Thi

SUBMITTER: Chevallet M 

PROVIDER: S-EPMC2797540 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature