Nanostructural and transcriptomic analyses of human saliva derived exosomes.

ABSTRACT:

Background

Exosomes, derived from endocytic membrane vesicles are thought to participate in cell-cell communication and protein and RNA delivery. They are ubiquitous in most body fluids (breast milk, saliva, blood, urine, malignant ascites, amniotic, bronchoalveolar lavage, and synovial fluids). In particular, exosomes secreted in human saliva contain proteins and nucleic acids that could be exploited for diagnostic purposes. To investigate this potential use, we isolated exosomes from human saliva and characterized their structural and transcriptome contents.

Methodology

Exosomes were purified by differential ultracentrifugation and identified by immunoelectron microscopy (EM), flow cytometry, and Western blot with CD63 and Alix antibodies. We then described the morphology,

SUBMITTER: Palanisamy V 

PROVIDER: S-EPMC2797607 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature