Diet-induced gene expression of isolated pancreatic islets from a polygenic mouse model of the metabolic syndrome.

ABSTRACT:

Aims/hypothesis

Numerous new genes have recently been identified in genome-wide association studies for type 2 diabetes. Most are highly expressed in beta cells and presumably play important roles in their function. However, these genes account for only a small proportion of total risk and there are likely to be additional candidate genes not detected by current methodology. We therefore investigated islets from the polygenic New Zealand mouse (NZL) model of diet-induced beta cell dysfunction to identify novel genes and pathways that may play a role in the pathogenesis of diabetes.

Methods

NZL mice were fed a diabetogenic high-fat diet (HF) or a diabetes-protective carbohydrate-free HF diet (CHF). Pancreatic islets were isolated by laser capture microdissection (LCM) and sub

SUBMITTER: Dreja T 

PROVIDER: S-EPMC2797618 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature