Carbohydrate-responsive element-binding protein (ChREBP) is a negative regulator of ARNT/HIF-1beta gene expression in pancreatic islet beta-cells.

ABSTRACT:

Objective

Carbohydrate-responsive element-binding protein (ChREBP) is a transcription factor that has been shown to regulate carbohydrate metabolism in the liver and pancreatic beta-cells in response to elevated glucose concentrations. Because few genes have been identified so far as bona fide ChREBP-target genes, we have performed a genome-wide analysis of the ChREBP transcriptome in pancreatic beta-cells.

Research design and methods

Chromatin immunoprecipitation and high-density oligonucleotide tiling arrays (ChIP-chip; Agilent Technologies) using MIN6 pancreatic beta-cell extracts were performed together with transcriptional and other analysis using standard techniques.

Results

One of the genes identified by ChIP-chip and linked to glucose sensing and insulin secre

SUBMITTER: Noordeen NA 

PROVIDER: S-EPMC2797916 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature