Proteomics analysis of human skeletal muscle reveals novel abnormalities in obesity and type 2 diabetes.

ABSTRACT:

Objective

Insulin resistance in skeletal muscle is an early phenomenon in the pathogenesis of type 2 diabetes. Studies of insulin resistance usually are highly focused. However, approaches that give a more global picture of abnormalities in insulin resistance are useful in pointing out new directions for research. In previous studies, gene expression analyses show a coordinated pattern of reduction in nuclear-encoded mitochondrial gene expression in insulin resistance. However, changes in mRNA levels may not predict changes in protein abundance. An approach to identify global protein abundance changes involving the use of proteomics was used here.

Research design and methods

Muscle biopsies were obtained basally from lean, obese, and type 2 diabetic volunteers (n = 8 each);

SUBMITTER: Hwang H 

PROVIDER: S-EPMC2797941 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature