Virulence, inflammatory potential, and adaptive immunity induced by Shigella flexneri msbB mutants.

ABSTRACT: The ability of genetically detoxified lipopolysaccharide (LPS) to stimulate adaptive immune responses is an ongoing area of investigation with significant consequences for the development of safe and effective bacterial vaccines and adjuvants. One approach to genetic detoxification is the deletion of genes whose products modify LPS. The msbB1 and msbB2 genes, which encode late acyltransferases, were deleted in the Shigella flexneri 2a human challenge strain 2457T to evaluate the virulence, inflammatory potential, and acquired immunity induced by strains producing underacylated lipid A. Consistent with a reduced endotoxic potential, S. flexneri 2a msbB mutants were attenuated in an acute mouse pulmonary challenge model. Attenuation correlated with decreases in the production of proinflammat

SUBMITTER: Ranallo RT 

PROVIDER: S-EPMC2798193 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature