Transcriptional and functional analysis of the Neisseria gonorrhoeae Fur regulon.

ABSTRACT: To ensure survival in the host, bacteria have evolved strategies to acquire the essential element iron. In Neisseria gonorrhoeae, the ferric uptake regulator Fur regulates metabolism through transcriptional control of iron-responsive genes by binding conserved Fur box (FB) sequences in promoters during iron-replete growth. Our previous studies showed that Fur also controls the transcription of secondary regulators that may, in turn, control pathways important to pathogenesis, indicating an indirect role for Fur in controlling these downstream genes. To better define the iron-regulated cascade of transcriptional control, we combined three global strategies--temporal transcriptome analysis, genomewide in silico FB prediction, and Fur titration assays (FURTA)--to detect genomic regions able t

SUBMITTER: Jackson LA 

PROVIDER: S-EPMC2798271 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature