Ontology highlight
ABSTRACT:
SUBMITTER: Du J
PROVIDER: S-EPMC2798731 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Du Jianhai J Wei Na N Xu Hao H Ge Ying Y Vásquez-Vivar Jeannette J Guan Tongju T Oldham Keith T KT Pritchard Kirkwood A KA Shi Yang Y
Arteriosclerosis, thrombosis, and vascular biology 20090917 12
<h4>Objective</h4>The posttranslational regulation of GTP cyclohydrolase I (GCH-1), the rate-limiting enzyme for tetrahydrobiopterin (BH4) synthesis, remains elusive. Here, we identified specific phosphorylation sites on GCH-1 and characterized the function of these sites.<h4>Methods and results</h4>Mass spectrometry studies showed overexpressed rat GCH-1 was phosphorylated at serine (S) 51, S167, and threonine (T) 231 in HEK293 cells, whereas a computational analysis of GCH-1 revealed 8 potenti ...[more]