Mutational insertion of a ROSA26-EGFP transgene leads to defects in spermiogenesis and male infertility in mice.

ABSTRACT: Pronuclear injection has been a successful strategy for generating genetically engineered mouse models to better understand the functionality of genes. A characteristic of pronuclear injection is that random integration of the transgene into the genome can disturb a functional gene and result in a phenotype unrelated to the transgene itself. In this study, we have characterized a mouse model containing an insertional mutation that, in the homozygous state, severely affects spermatogenesis as characterized by lack of sperm motility and acrosomal aplasia. Whereas homozygous female mice had normal fertility, male mice homozygous for the insertional mutation were unable to produce pups by natural mating with either homozygous or wild-type female mice. No fertilized embryos were produced by mat

SUBMITTER: Walters EM 

PROVIDER: S-EPMC2798843 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature