Systematic characterization of the murine mitochondrial proteome using functionally validated cardiac mitochondria.

ABSTRACT: Mitochondria play essential roles in cardiac pathophysiology and the murine model has been extensively used to investigate cardiovascular diseases. In the present study, we characterized murine cardiac mitochondria using an LC/MS/MS approach. We extracted and purified cardiac mitochondria; validated their functionality to ensure the final preparation contains necessary components to sustain their normal function; and subjected these validated organelles to LC/MS/MS-based protein identification. A total of 940 distinct proteins were identified from murine cardiac mitochondria, among which, 480 proteins were not previously identified by major proteomic profiling studies. The 940 proteins consist of functional clusters known to support oxidative phosphorylation, metabolism, and biogenesis. In

SUBMITTER: Zhang J 

PROVIDER: S-EPMC2799225 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature