Promoter methylation of IGFBP-3 and p53 expression in ovarian endometrioid carcinoma.

ABSTRACT:

Background

Insulin-like growth factor binding protein (IGFBP-3) is an antiproliferative, pro-apoptotic and invasion suppressor protein which is transcriptionally regulated by p53. Promoter methylation has been linked to gene silencing and cancer progression. We studied the correlation between IGFBP-3 and p53 expression as well as IGFBP-3 promoter methylation in ovarian endometrioid carcinoma (OEC) by immunohistochemical staining and quantitative methylation-specific PCR (qMSP). Additionally, we assessed the molecular regulatory mechanism of wild type (wt) p53 on IGFBP-3 expression using two subclones of OEC, the OVTW59-P0 (low invasive) and P4 (high invasive) sublines.

Results

In 60 cases of OEC, 40.0% showed lower IGFBP-3 expression which was significantly correlated with h

SUBMITTER: Torng PL 

PROVIDER: S-EPMC2799391 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature