Pyrosequencing identification of Mycobacterium tuberculosis W-Beijing.

ABSTRACT:

Background

The worldwide expanding Mycobacterium tuberculosis W-Beijing family is associated with treatment failure and relapse. Its identification currently relies on spoligotyping and conventional sequencing. We developed pyrosequencing as an alternative method for its identification.

Findings

Pyrosequencing found a G/A substitution in the Rv0927c-pstS3 intergenic spacer and a RD105 deletion, identifying 8/104 M. tuberculosis isolates as W-Beijing isolates. In addition, pyrosequencing found a previously unreported TGC deletion in the Rv0927c gene of W-Beijing isolates. Total concordance was found between the pyrosequencing data and conventional sequencing, as well as reference molecular identification. Multispacer Sequence Typing assigned the W-Beijing isolates to the Asia

SUBMITTER: Djelouadji Z 

PROVIDER: S-EPMC2799434 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature