Application of hybridization control probe to increase accuracy on ligation detection or minisequencing diagnostic microarrays.

ABSTRACT:

Background

Nucleic acid detection based on ligation reaction or single nucleotide extension of ssDNA probes followed by tag microarray hybridization provides an accurate and sensitive detection tool for various diagnostic purposes. Since microarray quality is crucial for reliable detection, these methods can benefit from correcting for microarray artefacts using specifically adapted techniques.

Findings

Here we demonstrate the application of a per-spot hybridization control oligonucleotide probe and a novel way of computing normalization for tag array data. The method takes into account the absolute value of the detection probe signal and the variability in the control probe signal to significantly alleviate problems caused by artefacts and noise on low quality microarrays.<

SUBMITTER: Ritari J 

PROVIDER: S-EPMC2799435 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature