Exon-level expression profiling: a comprehensive transcriptome analysis of oral fluids.

ABSTRACT:

Background

The application of global gene expression profiling to saliva samples is hampered by the presence of partially fragmented and degraded RNAs that are difficult to amplify and detect with the prevailing technologies. Moreover, the often limited volume of saliva samples is a challenge to quantitative PCR (qPCR) validation of multiple candidates. The aim of this study was to provide proof-of-concept data on the combination of a universal mRNA-amplification method with exon arrays for candidate selection and a multiplex preamplification method for easy validation.

Methods

We used a universal mRNA-specific linear-amplification strategy in combination with Affymetrix Exon Arrays to amplify salivary RNA from 18 healthy individuals on the nanogram scale. Multiple selected

SUBMITTER: Hu Z 

PROVIDER: S-EPMC2799536 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature