C.1810C>T polymorphism of NTRK1 gene is associated with reduced survival in neuroblastoma patients.

ABSTRACT:

Background

TrkA (encoded by NTRK1 gene), the high-affinity tyrosine kinase receptor for neurotrophins, is involved in neural crest cell differentiation. Its expression has been reported to be associated with a favourable prognosis in neuroblastoma. Therefore, the entire coding sequence of NTRK1 gene has been analysed in order to identify mutations and/or polymorphisms which may alter TrkA receptor expression.

Methods

DNA was extracted from neuroblastomas of 55 Polish and 114 Italian patients and from peripheral blood leukocytes of 158 healthy controls. Denaturing High-Performance Liquid Chromatography (DHPLC) and Single-Strand Conformation Polymorphism (SSCP) analysis were used to screen for sequence variants. Genetic changes were confirmed by direct sequencing and correlate

SUBMITTER: Lipska BS 

PROVIDER: S-EPMC2800120 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature