Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca).

ABSTRACT: Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo. The clinical disease profile suggested that the pandas may have suffered a viral infection. Therefore, a series of detection including virus isolation, electron microscopy, cytobiological assay, serum neutralization and RT-PCR were used to identify the virus. It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV. The results suggest that the affected pandas had been infected with CCV.

SUBMITTER: Gao FS 

PROVIDER: S-EPMC2801125 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature