An improved phage-display panning method to produce an HM-1 killer toxin anti-idiotypic antibody.

ABSTRACT:

Background

Phage-display panning is an integral part of biomedical research. Regular panning methods are sometimes complicated by inefficient detachment of the captured phages from the antigen-coated solid supports, which prompted us to modify. Here, we produce an efficient antigen-specific single chain fragment variable (scFv) antibody by using a target-related molecule that favored selection of recombinant antibodies.

Results

To produce more selective and specific anti-idiotypic scFv-antibodies from a cDNA library, constructed from HM-1 killer toxin (HM-1)-neutralizing monoclonal antibodies (nmAb-KT), the method was modified by using an elution buffer supplemented with HM-1 that shares structural and functional similarities with the active site of the scFv antibody. Compet

SUBMITTER: Kabir ME 

PROVIDER: S-EPMC2801674 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature