Role of FRNK tyrosine phosphorylation in vascular smooth muscle spreading and migration.

ABSTRACT:

Aims

Focal adhesion kinase (FAK) and its autonomously expressed, C-terminal inhibitor FAK-related non-kinase (FRNK), are important regulators of vascular smooth muscle cell (VSMC) spreading and migration. However, the mechanisms of FRNK-mediated inhibition of FAK-dependent signalling are not fully defined. The aim of this study was to determine the potential role of FRNK tyrosine phosphorylation in regulating these processes.

Methods and results

Rat carotid arteries were balloon-injured and FAK and FRNK expression and phosphorylation were examined by immunocytochemistry, immunoprecipitation, and western blotting with total and phosphospecific antibodies. FAK and FRNK expression increased four- and nine-fold, respectively, in alpha-smooth muscle actin-positive VSMCs of injure

SUBMITTER: Koshman YE 

PROVIDER: S-EPMC2802202 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature