Properties of a tobacco DNA methyltransferase, NtMET1 and its involvement in chromatin movement during cell division.

ABSTRACT:

Background and aims

Plants possess three types of DNA methyltransferase, among which methyltransferase type 1 (MET1) is considered to play a major role by maintaining the CpG methylation patterns. However, little information is available as to its enzymatic activity, interacting proteins and spatial and temporal behaviours during DNA replication. In the present study, one example, NtMET1 from tobacco plants, was selected and an analysis was made of its biochemical properties and cellular localization.

Methods

NtMET1 was expressed in Sf9 insect cells, and a purified sample was subjected to a standard in vitro methylation assay. Intramolecular interaction was examined by the yeast two-hybrid and pull-down assays. Transgenic tobacco plants (Nicotiana tabacum) over-expressing Nt

SUBMITTER: Kim HJ 

PROVIDER: S-EPMC2802912 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature