Ontology highlight
ABSTRACT:
SUBMITTER: Fabian MR
PROVIDER: S-EPMC2803087 | biostudies-literature | 2009 Sep
REPOSITORIES: biostudies-literature
Molecular cell 20090827 6
MicroRNAs (miRNAs) inhibit mRNA expression in general by base pairing to the 3'UTR of target mRNAs and consequently inhibiting translation and/or initiating poly(A) tail deadenylation and mRNA destabilization. Here we examine the mechanism and kinetics of miRNA-mediated deadenylation in mouse Krebs-2 ascites extract. We demonstrate that miRNA-mediated mRNA deadenylation occurs subsequent to initial translational inhibition, indicating a two-step mechanism of miRNA action, which serves to consoli ...[more]