A systems analysis of the chemosensitivity of breast cancer cells to the polyamine analogue PG-11047.

ABSTRACT:

Background

Polyamines regulate important cellular functions and polyamine dysregulation frequently occurs in cancer. The objective of this study was to use a systems approach to study the relative effects of PG-11047, a polyamine analogue, across breast cancer cells derived from different patients and to identify genetic markers associated with differential cytotoxicity.

Methods

A panel of 48 breast cell lines that mirror many transcriptional and genomic features present in primary human breast tumours were used to study the antiproliferative activity of PG-11047. Sensitive cell lines were further examined for cell cycle distribution and apoptotic response. Cell line responses, quantified by the GI50 (dose required for 50% relative growth inhibition) were correlated with the

SUBMITTER: Kuo WL 

PROVIDER: S-EPMC2803786 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature