Cloning, identification, and characterization of the rpoS-like sigma factor rpoX from Vibrio alginolyticus.

ABSTRACT: Vibrio alginolyticus ZJ-51 displays phase variation between opaque/rugose colonies (Op) and translucent/smooth colonies (Tr). These colony variants show great differences in biofilm formation and motility. In this study, a gene encoding for an rpoS-like sigma factor, rpoX, has been cloned and characterized. The absence of rpoX did not affect colony switching rate but did decrease biofilm formation in both the Op and the Tr variants. When challenged with hydrogen peroxide, the DeltarpoX in the Op background showed a slightly higher survival rate compared with the wild type, whereas survival was decreased in the Tr background. Deletion of rpoX in the Tr background resulted in a higher ability to resist ethanol challenges and to survive hyperosmolarity challenges, and in the Op background the

SUBMITTER: Zhao JJ 

PROVIDER: S-EPMC2804039 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature