Characterization of Ca2+-binding protein 5 knockout mouse retina.

ABSTRACT:

Purpose

The goal of this study was to investigate, with the use of CaBP5 knockout mice, whether Ca(2+)-binding protein 5 (CaBP5) is required for vision. The authors also tested whether CaBP5 can modulate expressed Ca(v)1.2 voltage-activated calcium channels.

Methods

CaBP5 knockout (Cabp5(-/-)) mice were generated. The retinal morphology and visual function of 6-week-old Cabp5(-/-) mice were analyzed by confocal and electron microscopy, single-flash electroretinography, and whole-cell patch-clamp recordings of retinal ganglion cells. The interaction and modulation of Ca(v)1.2 channels by CaBP5 were analyzed using affinity chromatography, gel overlay assays, and patch-clamp recordings of transfected HEK293 cells.

Results

No evidence of morphologic changes and no signifi

SUBMITTER: Rieke F 

PROVIDER: S-EPMC2804972 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature