Effects of technological processes on the tenacity and inactivation of norovirus genogroup II in experimentally contaminated foods.

ABSTRACT: Contaminated food is a significant vehicle for human norovirus transmission. The present study determined the effect of physicochemical treatments on the tenacity of infective human norovirus genogroup II in selected foods. Artificially contaminated produce was subjected to a number of processes used by the food industry for preservation and by the consumer for storage and preparation. Virus recovery was carried out by using ultrafiltration and was monitored by using bacteriophage MS2 as an internal process control. Norovirus was quantified by using monoplex one-step TaqMan real-time reverse transcription (RT)-PCR and an external standard curve based on recombinant RNA standards. An RNase pretreatment step was used to avoid false-positive PCR results caused by accessible RNA, which allowed

SUBMITTER: Mormann S 

PROVIDER: S-EPMC2805205 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature