Identification and partial characterization of a dynamin-like protein, EhDLP1, from the protist parasite Entamoeba histolytica.

ABSTRACT: The dynamin superfamily of proteins includes a large repertoire of evolutionarily conserved GTPases that interact with different subcellular organelle membranes in eukaryotes. Dynamins are thought to participate in a number of cellular processes involving membrane remodeling and scission. Dynamin-like proteins (DLPs) form a subfamily of this vast class and play important roles in cellular processes, such as mitochondrial fission, cytokinesis, and endocytosis. In the present study, a gene encoding a dynamin-like protein (EhDLP1) from the protist parasite Entamoeba histolytica was identified and the protein was partially characterized using a combination of in silico, biochemical, and imaging methods. The protein was capable of GTP binding and hydrolysis, lipid binding, and oligomerization.

SUBMITTER: Jain R 

PROVIDER: S-EPMC2805295 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature