Bistable expression of CsgD in biofilm development of Salmonella enterica serovar typhimurium.

ABSTRACT: Bacterial persistence in the environment and in the infected host is often aided by the formation of exopolymer-enclosed communities known as biofilms. Heterogeneous gene expression takes place in microcompartments formed within the complex biofilm structure. This study describes cell differentiation within an isogenic bacterial cell population based on the example of biofilm formation by Salmonella enterica serovar Typhimurium. We analyzed the expression of the major biofilm regulator CsgD at the single-cell level with a chromosomal CsgD-green fluorescent protein (GFP) translational fusion. In individual cells, CsgD-GFP expression is mostly found in the cytoplasm. Quantitative expression analysis and results from three different models of S. Typhimurium biofilms demonstrated that CsgD is

SUBMITTER: Grantcharova N 

PROVIDER: S-EPMC2805326 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature