Copia and Gypsy retrotransposons activity in sunflower (Helianthus annuus L.).

ABSTRACT:

Background

Retrotransposons are heterogeneous sequences, widespread in eukaryotic genomes, which refer to the so-called mobile DNA. They resemble retroviruses, both in their structure and for their ability to transpose within the host genome, of which they make up a considerable portion. Copia- and Gypsy-like retrotransposons are the two main classes of retroelements shown to be ubiquitous in plant genomes. Ideally, the retrotransposons life cycle results in the synthesis of a messenger RNA and then self-encoded proteins to process retrotransposon mRNA in double stranded extra-chromosomal cDNA copies which may integrate in new chromosomal locations.

Results

The RT-PCR and IRAP protocol were applied to detect the presence of Copia and Gypsy retrotransposon transcripts and of

SUBMITTER: Vukich M 

PROVIDER: S-EPMC2805666 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature