Genes targeted by the estrogen and progesterone receptors in the human endometrial cell lines HEC1A and RL95-2.

ABSTRACT:

Background

When the steroid hormones estrogen and progesterone bind to nuclear receptors, they have transcriptional impact on target genes in the human endometrium. These transcriptional changes have a critical function in preparing the endometrium for embryo implantation.

Methods

382 genes were selected, differentially expressed in the receptive endometrium, to study their responsiveness of estrogen and progesterone. The endometrial cell lines HEC1A and RL95-2 were used as experimental models for the non-receptive and receptive endometrium, respectively. Putative targets for activated steroid hormone receptors were investigated by chromatin immunoprecipitation (ChIP) using receptor-specific antibodies. Promoter occupancy of the selected genes by steroid receptors was detect

SUBMITTER: Tamm K 

PROVIDER: S-EPMC2805670 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature