Generation and analysis of expression sequence tags from haustoria of the wheat stripe rust fungus Puccinia striiformis f. sp. Tritici.

ABSTRACT:

Background

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. In spite of its agricultural importance, the genomics and genetics of the pathogen are poorly characterized. Pst transcripts from urediniospores and germinated urediniospores have been examined previously, but little is known about genes expressed during host infection. Some genes involved in virulence in other rust fungi have been found to be specifically expressed in haustoria. Therefore, the objective of this study was to generate a cDNA library to characterize genes expressed in haustoria of Pst.

Results

A total of 5,126 EST sequences of high quality were generated from haustoria of Pst, from which 287 contigs and

SUBMITTER: Yin C 

PROVIDER: S-EPMC2805700 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature