P300 alters keratinocyte cell growth and differentiation through regulation of p21(Waf1/CIP1).

ABSTRACT:

Background

p300 functions as a transcriptional co-activator to regulate many cellular responses such as cell growth, transformation, development and differentiation. It has been shown to affect the transcriptional activity of p53 which regulates p21(Waf1/CIP1) expression, however, the role of p300 in differentiation remains unclear.

Methodology and principal findings

Knockdown of p300 protein with short hairpin RNA (shRNA) molecules delays human neonatal foreskin keratinocyte (HFKs) differentiation. Moreover, depletion of p300 increases the proliferative capacity of HFKs, extends the life span of cells and allows differentiated HFKs to re-enter the cell cycle. Studies indicate that depletion of p300 down-regulates the acetylation and expression of p53, and chromatin immunopr

SUBMITTER: Wong PP 

PROVIDER: S-EPMC2805707 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature