A rhodanine agent active against non-replicating intracellular Mycobacterium avium subspecies paratuberculosis.

ABSTRACT:

Background

Antibiotic therapy targeting chronic mycobacterial disease is often ineffective due to problems with the emergence of drug resistance and non-replicating persistent intracellular antibiotic resistant phenotypes. Strategies which include agents able to enhance host cell killing mechanisms could represent an alternative to conventional methods with the potential for host clearance if active against dormant phenotypes. Investigations of agents with potential activity against non-replicating mycobacteria however are restricted due to a need for assays that can assess bacterial viability without having to culture.

Results

This study describes the development and use of a pre16S ribosomal gene RNA/DNA ratio viability assay which is independent of the need for culture, s

SUBMITTER: Bull TJ 

PROVIDER: S-EPMC2806251 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature