Single strand conformation polymorphism based SNP and Indel markers for genetic mapping and synteny analysis of common bean (Phaseolus vulgaris L.).

ABSTRACT:

Background

Expressed sequence tags (ESTs) are an important source of gene-based markers such as those based on insertion-deletions (Indels) or single-nucleotide polymorphisms (SNPs). Several gel based methods have been reported for the detection of sequence variants, however they have not been widely exploited in common bean, an important legume crop of the developing world. The objectives of this project were to develop and map EST based markers using analysis of single strand conformation polymorphisms (SSCPs), to create a transcript map for common bean and to compare synteny of the common bean map with sequenced chromosomes of other legumes.

Results

A set of 418 EST based amplicons were evaluated for parental polymorphisms using the SSCP technique and 26% of these present

SUBMITTER: Galeano CH 

PROVIDER: S-EPMC2806352 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature