Ontology highlight
ABSTRACT:
SUBMITTER: Song Y
PROVIDER: S-EPMC2806505 | biostudies-literature | 2010 Jan
REPOSITORIES: biostudies-literature
Song Y Y Bahnson A A Hall N N Yu H H Shen H H Koebler D D Houck R R Xie Y Y Cheng T T
Leukemia 20091001 1
A deeper understanding of stem cell niche engagement and subsequent behaviors would be enhanced by technologies enabling the tracking of individual stem cells at the clonal level in long-term co-culture (LTC), which mimics the complexity of the bone marrow microenvironment in vivo. Here, we report the application of time-lapse imaging with intermittent fluorescence for tracking well-defined populations of GFP(+) murine hematopoietic stem cells (HSCs) using LTC for >5 weeks. Long-term (LT) and sh ...[more]