Sequencing newly replicated DNA reveals widespread plasticity in human replication timing.

ABSTRACT: Faithful transmission of genetic material to daughter cells involves a characteristic temporal order of DNA replication, which may play a significant role in the inheritance of epigenetic states. We developed a genome-scale approach--Repli Seq--to map temporally ordered replicating DNA using massively parallel sequencing and applied it to study regional variation in human DNA replication time across multiple human cell types. The method requires as few as 8,000 cytometry-fractionated cells for a single analysis, and provides high-resolution DNA replication patterns with respect to both cell-cycle time and genomic position. We find that different cell types exhibit characteristic replication signatures that reveal striking plasticity in regional replication time patterns covering at least 5

SUBMITTER: Hansen RS 

PROVIDER: S-EPMC2806781 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature