The MCM-binding protein ETG1 aids sister chromatid cohesion required for postreplicative homologous recombination repair.

ABSTRACT: The DNA replication process represents a source of DNA stress that causes potentially spontaneous genome damage. This effect might be strengthened by mutations in crucial replication factors, requiring the activation of DNA damage checkpoints to enable DNA repair before anaphase onset. Here, we demonstrate that depletion of the evolutionarily conserved minichromosome maintenance helicase-binding protein ETG1 of Arabidopsis thaliana resulted in a stringent late G2 cell cycle arrest. This arrest correlated with a partial loss of sister chromatid cohesion. The lack-of-cohesion phenotype was intensified in plants without functional CTF18, a replication fork factor needed for cohesion establishment. The synergistic effect of the etg1 and ctf18 mutants on sister chromatid cohesion strengthened t

SUBMITTER: Takahashi N 

PROVIDER: S-EPMC2806904 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature