The malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesin.

ABSTRACT:

Background

The pathogenic fungus Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis (PCM). This is a pulmonary mycosis acquired by inhalation of fungal airborne propagules that can disseminate to several organs and tissues leading to a severe form of the disease. Adhesion and invasion to host cells are essential steps involved in the internalization and dissemination of pathogens. Inside the host, P. brasiliensis may use the glyoxylate cycle for intracellular survival.

Results

Here, we provide evidence that the malate synthase of P. brasiliensis (PbMLS) is located on the fungal cell surface, and is secreted. PbMLS was overexpressed in Escherichia coli, and polyclonal antibody was obtained against this protein. By using Confocal Laser Scanning Microscopy, Pb

SUBMITTER: da Silva Neto BR 

PROVIDER: S-EPMC2807876 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature