Sympatric ecological speciation meets pyrosequencing: sampling the transcriptome of the apple maggot Rhagoletis pomonella.

ABSTRACT:

Background

The full power of modern genetics has been applied to the study of speciation in only a small handful of genetic model species--all of which speciated allopatrically. Here we report the first large expressed sequence tag (EST) study of a candidate for ecological sympatric speciation, the apple maggot Rhagoletis pomonella, using massively parallel pyrosequencing on the Roche 454-FLX platform. To maximize transcript diversity we created and sequenced separate libraries from larvae, pupae, adult heads, and headless adult bodies.

Results

We obtained 239,531 sequences which assembled into 24,373 contigs. A total of 6810 unique protein coding genes were identified among the contigs and long singletons, corresponding to 48% of all known Drosophila melanogaster protein-co

SUBMITTER: Schwarz D 

PROVIDER: S-EPMC2807884 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature