Helicobacter pylori HopE and HopV porins present scarce expression among clinical isolates.

ABSTRACT:

Aim

To evaluate how widely Helicobacter pylori (H. pylori) HopE and HopV porins are expressed among Chilean isolates and how seroprevalent they are among infected patients in Chile.

Methods

H. pylori hopE and hopV genes derived from strain CHCTX-1 were cloned by polymerase chain reaction (PCR), sequenced and expressed in Escherichia coli AD494 (DE3). Gel-purified porins were used to prepare polyclonal antibodies. The presence of both genes was tested by PCR in a collection of H. pylori clinical isolates and their expression was detected in lysates by immunoblotting. Immune responses against HopE, HopV and other H. pylori antigens in sera from infected and non-infected patients were tested by Western blotting using these sera as first antibody on recombinant H. pylori antigen

SUBMITTER: Lienlaf M 

PROVIDER: S-EPMC2807952 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature