Automated detection of conformational epitopes using phage display Peptide sequences.

ABSTRACT:

Background

Precise determination of conformational epitopes of neutralizing antibodies represents a key step in the rational design of novel vaccines. A powerful experimental method to gain insights on the physical chemical nature of conformational epitopes is the selection of linear peptides that bind with high affinities to a monoclonal antibody of interest by phage display technology. However, the structural characterization of conformational epitopes from these mimotopes is not straightforward, and in the past the interpretation of peptide sequences from phage display experiments focused on linear sequence analysis to find a consensus sequence or common sequence motifs.

Results

We present a fully automated search method, EpiSearch that predicts the possible location of c

SUBMITTER: Negi SS 

PROVIDER: S-EPMC2808184 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature