Gene-targeted-metagenomics reveals extensive diversity of aromatic dioxygenase genes in the environment.

ABSTRACT: Understanding the relationship between gene diversity and function for important environmental processes is a major ecological research goal. We applied gene-targeted metagenomics and pyrosequencing to aromatic dioxygenase genes to obtain greater sequence depth than possible by other methods. A polymerase chain reaction (PCR) primer set designed to target a 524-bp region that confers substrate specificity of biphenyl dioxygenases yielded 2000 and 604 sequences from the 5' and 3' ends of PCR products, respectively, which passed our validity criteria. Sequence alignment showed three known conserved residues, as well as another seven conserved residues not reported earlier. Of the valid sequences, 95% and 41% were assigned to 22 and 3 novel clusters in that they did not include any earlier re

SUBMITTER: Iwai S 

PROVIDER: S-EPMC2808446 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature