Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.

ABSTRACT:

Background

Deconjugation of ubiquitin and/or ubiquitin-like modified protein substrates is essential to modulate protein-protein interactions and, thus, signaling processes in cells. Although deubiquitylating (deubiquitinating) enzymes (DUBs) play a key role in this process, however, their function and regulation remain insufficiently understood. The "loss-of-function" phenotype studies can provide important information to elucidate the gene function, and zebrafish is an excellent model for this goal.

Results

From an in silico genome-wide search, we found more than 90 putative DUBs encoded in the zebrafish genome belonging to six different subclasses. Out of them, 85 from five classical subclasses have been tested with morpholino (MO) knockdown experiments and 57 of them wer

SUBMITTER: Tse WK 

PROVIDER: S-EPMC2809080 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature