Synthesis and characterization of biodegradable HPMA-oligolysine copolymers for improved gene delivery.

ABSTRACT: Bioactive peptides, including DNA-binding, endosomal release, and cell targeting peptides, have been integrated into synthetic gene carriers to improve delivery efficiency by enabling the vectors to overcome barriers to gene delivery. Our overall goal is to develop multifunctional, peptide-based polymers that incorporate motifs to condense DNA and facilitate sequential trafficking steps. One approach is to polymerize vinyl-terminated peptides by radical polymerization. In this work, cationic oligolysine peptides were designed to contain vinyl termini with internal reducible linkers. These peptides were copolymerized with HPMA to form biodegradable, DNA-condensing copolymers for gene delivery. The polymerization conditions were optimized by varying the initiator to monomer ratios, macromono

SUBMITTER: Burke RS 

PROVIDER: S-EPMC2809123 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature